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Organoids have specific advantages compared to single type of cells, after epidermal organoids were uniformly sprayed on the surface of the wound regions, the efficiency of wound healing was significantly enhanced. Remarkably, the passaged epidermal organoids contained multiple maturational lineage stages of epidermal cells, including expanded stem/progenitors, and relatively mature epidermal cells. After spray delivery onto the injured sites of a murine model, the expanded epidermal organoids efficiently integrated into the opened skin tissue, and improved skin reconstitution and regeneration.
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