stemInduce ™ MAG
Umbilical Cord Mesenchymal Stem Cell Assembly

The Intrinsic properties of many types of cells are critically altered when cell culture condition shifts from two-dimensional (2D) to three-dimensional (3D) environment. Currently, several lines of evidence have demonstrated the therapeutic potential of mesenchymal stem cells (MSCs) in regenerative medicine [1].

The advantages of 3D grown MSC include the promotion of self-renewal and differentiation activities as well as up-regulation of paracrine secretion [1]. Following the development of 3D cell culture, the enhanced therapeutic efficacy of spheroid-forming MSCs have been identified in several animal disease models by promoting differentiation or trophic factor secretion, as compared to planarcultured MSCs [2].

Spheroids are produced using different techniques such as hanging drop or low attachment surfaces. These techniques rely on the
cells attaching to each other by the lack of another alternative. So, in this scaffold-free platform, cells are organized into the cell
spheroids and floated in the culture medium due to the lack of tangible supporting materials [2].

In contrast to that, the medium stemInduce ™ MAG from SMSbiotech induces the active reorganization of umbilical cord mesenchymal stem cells that grown on a regular cell adherent plate. The cells migrate towards a center and organize into a complex 3D cell assembly. The initiation of this complex organization follows immediately after 2D cell confluency and continues for many days [3]

References:
1-Han, H.-W.; Asano, S.; Hsu, S.-H. Cellular Spheroids of Mesenchymal Stem Cells and Their Perspectives in Future Healthcare. Appl. Sci. 2019, 9, 627.
2-Sart S1, Tsai AC, Li Y, Ma T. Three-dimensional aggregates of mesenchymal stem cells: cellular mechanisms, biological properties, and applications. Tissue Eng Part B Rev. 2014 Oct;20(5):365-80.
3-Adamzyk C, Labude N, Schneider RK, et al. Human umbilical cord-derived mesenchymal stem cells spontaneously form 3d aggregates and differentiate in an embryoid body-like manner. J Stem Cell Res Ther. 2016;1(5):209‒216.